Targeted Genotyping Panel Ordering System Step 1 of 5 20% Contacts (required) Add one name and email for each contact type. Project Coordinator(s)NameEmailMarker Design Contact(s) if not Project CoordinatorSame as Project Coordinator? NameEmailContact(s) for receiving product name and custom code if not Project Coordinator Same as Project Coordinator? NameEmailContact(s) for the TG Positive Control (PC) and Validation Service if not Project CoordinatorSame as Project Coordinator? NameEmail Please provide here the general specifications for your required marker set. The marker design process produces various quality parameters based on flanking sequence composition, and target region copy number (if a Reference genome is available). These quality parameters will be used, in combination with optional values provided in the EssentialMarker, MinorAlleleFrequency, and Quality columns to choose a set of markers to make up the panel. When an optimal selection is required, providing an excess of suitable candidate markers will improve the outcome of the marker selection process. Optionally, if requested, marker design and design quality determination can be performed on the full set of candidate markers, and then returned to you to perform your own final selection of the optimal marker subset for panel creation. Please specify below how you would like the marker selection to be performed. For example, when marker position information is available, markers can be selected to provide an optimal spread across the genome, in addition to meeting specific quality criteria. SpecificationsAdditional details Total number of markers required.Number of candidate markers provided in submission file.Number of DArTseq candidate markers provided:Please provide an estimate of the number of samples you expect to genotype using this panel over the next 3 yearsReference genome download links(for any reference genome listed in the submission file, provide a link here)Reference genome nameReference link Add Remove Shipping AddressFirst NameLast NameDepartmentCompany/InstitutionAddress Street Address Address Line 2 City State / Province / Region ZIP / Postal Code AfghanistanAlbaniaAlgeriaAmerican SamoaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBoliviaBonaire, Sint Eustatius and SabaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBrunei DarussalamBulgariaBurkina FasoBurundiCabo VerdeCambodiaCameroonCanadaCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos IslandsColombiaComorosCongoCongo, Democratic Republic of theCook IslandsCosta RicaCroatiaCubaCuraçaoCyprusCzechiaCôte d'IvoireDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEswatiniEthiopiaFalkland IslandsFaroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and McDonald IslandsHoly SeeHondurasHong KongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Democratic People's Republic ofKorea, Republic ofKuwaitKyrgyzstanLao People's Democratic RepublicLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacaoMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorth MacedoniaNorthern Mariana IslandsNorwayOmanPakistanPalauPalestine, State ofPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarRomaniaRussian FederationRwandaRéunionSaint BarthélemySaint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint MartinSaint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint MaartenSlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwedenSwitzerlandSyria Arab RepublicTaiwanTajikistanTanzania, the United Republic ofThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkmenistanTurks and Caicos IslandsTuvaluTürkiyeUS Minor Outlying IslandsUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUruguayUzbekistanVanuatuVenezuelaViet NamVirgin Islands, BritishVirgin Islands, U.S.Wallis and FutunaWestern SaharaYemenZambiaZimbabweÅland Islands Country PhoneEmail Billing AddressSame as shipping address? First NameLast NameDepartmentCompany/InstitutionAddress Street Address Address Line 2 City State / Province / Region ZIP / Postal Code AfghanistanAlbaniaAlgeriaAmerican SamoaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBoliviaBonaire, Sint Eustatius and SabaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBrunei DarussalamBulgariaBurkina FasoBurundiCabo VerdeCambodiaCameroonCanadaCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos IslandsColombiaComorosCongoCongo, Democratic Republic of theCook IslandsCosta RicaCroatiaCubaCuraçaoCyprusCzechiaCôte d'IvoireDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEswatiniEthiopiaFalkland IslandsFaroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuamGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and McDonald IslandsHoly SeeHondurasHong KongHungaryIcelandIndiaIndonesiaIranIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Democratic People's Republic ofKorea, Republic ofKuwaitKyrgyzstanLao People's Democratic RepublicLatviaLebanonLesothoLiberiaLibyaLiechtensteinLithuaniaLuxembourgMacaoMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMartiniqueMauritaniaMauritiusMayotteMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorth MacedoniaNorthern Mariana IslandsNorwayOmanPakistanPalauPalestine, State ofPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalPuerto RicoQatarRomaniaRussian FederationRwandaRéunionSaint BarthélemySaint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint MartinSaint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint MaartenSlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwedenSwitzerlandSyria Arab RepublicTaiwanTajikistanTanzania, the United Republic ofThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkmenistanTurks and Caicos IslandsTuvaluTürkiyeUS Minor Outlying IslandsUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUruguayUzbekistanVanuatuVenezuelaViet NamVirgin Islands, BritishVirgin Islands, U.S.Wallis and FutunaWestern SaharaYemenZambiaZimbabweÅland Islands Country Please upload relevant CSV below Drop files here or Select files Max. file size: 32 MB. Material Information and Requirements for TG Panel Positive control for TG panel The Positive Control (PC) is a genotype predicted to score for the majority of the markers in the panel and is required to be submitted with the validation service (or prior if preferred). The PC is used internally for DArTag assay QC purposes, with one PC for every 94 samples processed. For panels predicted to be used for thousands of samples, it is important to select the genotype which can be resubmitted as required. The Positive Control (PC) can be submitted either as already extracted gDNA or as tissue for extraction to be processed by DArT. PC submitted as gDNA We require a minimum of 500 µl of 50–100 ng/µl High Molecular Weight (HMW) and pure gDNA with quality suitable for DNA-modifying enzymes. If the panel is predicted to be used for thousands of samples, proportionally larger quantities of gDNA PC are required. gDNA PC can be submitted suspended in TE buffer, precipitated, or lyophilised. Any molecular-grade plastic container of suitable size may be used. The completed PC Specification Document – gDNA has to be included in the shipment alongside all other required documentation if shipped from overseas. PC Specification Document gDNA links the physical PC sample with the correct product and provides technical details on quantity. PC submitted as tissue The tissue quantity depends on the predicted number of samples to be processed on the panel. A few grams of tissue are required for DArT’s extraction process to provide sufficient PC in most use cases. Sample preparation and shipment depend on the organism and tissue type. Recommendations are available at: Plants, leaves, and seeds Samples from animals The completed PC Specification Document – Tissue has to be included in the shipment alongside all other required documentation if shipped from overseas. PC Specification Document Tissue links the physical PC sample with the correct product and provides technical details on quantity. TG validation service The validation service is a user-selected set of samples used to assess marker performance. Ordering the validation service and any follow-up services requires providing the product custom code. Ordering details will be provided to the specified contact via email. A minimum of 94 samples is required for panel validation; however, we recommend four plates with 94 samples. For use cases with fewer than 94 samples or more than four plates of 94 samples, please contact dart@diversityarrays.com. When preparing the shipment of material for product validation for DArTag products, please follow our recommendations specific to Targeted Genotyping (TG) services in addition to the general sample submission recommendations. These details are available on our website: General recommendations: https://help.diversityarrays.com/ Targeted Genotyping (TG) service recommendations: https://www.diversityarrays.com/services/targeted-genotying/ https://help.diversityarrays.com/docs/targeted-genotyping
