Gene tagging & cloning
Our gene tagging and cloning services enable better insights and help develop disease resistance for genes of interest.
Gene tagging & cloning
What is gene tagging?
Gene tagging involves more specific identification of the sequence of the DNA that is associated with a particular phenotype or trait. This is similar to QTL and GWAS.
What is gene cloning?
Cloning uses a different method again, to pinpoint a specific piece of DNA rather than individual markers. It works by studying causal factors at a molecular level, rather than by pure association.
Using Quantitative Bulk Segregant Analysis and Distribution Tails approaches, we can identify many tightly linked markers with a target gene. With enough gamete equivalents and availability of 100% accurate phenotyping, this can be extended to cloning the gene you are after.Â
Gene tagging can be used to target disease resistance in a range of crops and plants.Â
1 of 1Benefits and use-cases:
Gene tagging and cloning methods can be beneficial in:
- Identifying disease resistance genes
- Developing detailed understanding of trait genetics
- Establishing targets for genetic intervention (e.g. gene editing)
Case study
We have successfully used this approach to target disease resistance in pineapples, colour determination in custard apples, and leaf necrosis in maize.
